MY MENU

여름정기학술대회

2022여름초록

제목

Effect of mobile-phase composition and column on chromatographic separation of hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs)

작성자
강민식

발표자 및 발표 내용

소속
서강대학교/KIST
발표구분
포스터발표
포스터발표
3. Food & Environment
Brief Oral Presentation 발표신청
신청자에 한함
Keyword
Liquid Chromatography
Mass Spectrometry
Polycyclic aromatic hydrocarbons
Mobile phase composition
Column
Separation

주저자

이름
강민식
소속
서강대학교/KIST
국가
대한민국

공동저자

공동저자
이름
이강미
소속
KIST
국가
대한민국
이름
정태영
소속
서강대학교/KIST
국가
대한민국
이름
장희진
소속
서강대학교/KIST
국가
대한민국
이름
김민균
소속
서강대학교/KIST
국가
대한민국
이름
이재익
소속
KIST
국가
대한민국
이름
소속
국가
이름
소속
국가
이름
소속
국가
이름
소속
국가
이름
소속
국가

접수자

이름
강민식
소속
서강대학교/KIST

Polycyclic aromatic hydrocarbons (PAHs) are a wide range of environmental pollutants generated during the combustion of carbonaceous materials at high temperatures. It is a highly toxic substance with various side effects on human health when exposed. Therefore, a practical and sensitive analytical method for examining PAH exposure in humans is required. Monitoring human exposure to these requires observation of metabolites of biological samples. However, the current hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) analysis method using liquid chromatography-mass spectrometry (LC-MS/MS) suffers from low ionization efficiency and isomer separation. In particular, the problem of peak separation of many isomers must be optimized.

This study investigated the effects of mobile and stationary phase composition on peak separation and chromatographic behavior. First, the gradient program was optimized based on the ionization efficiency according to the organic solvent content. Next, peak separation patterns were investigated through various combinations of C18, AR, and pentafluorophenyl (PFP) columns as stationary phases and no additive, ammonium acetate, and ammonium fluoride as mobile phase additives. As a result, OH-NAP and OH-FLU were separated entirely, and OH-PHE was partially separated to achieve good chromatographic behavior. These results help optimize parameters such as mobile phase composition, column, and gradient when developing an OH-PAHs analysis method.

 

This study was supported by an intramural grant from Korea Institute of Science and Technology.

게시물수정

게시물 수정을 위해 비밀번호를 입력해주세요.

댓글삭제게시물삭제

게시물 삭제를 위해 비밀번호를 입력해주세요.