여름정기학술대회
2022여름초록
발표자 및 발표 내용
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Brief Oral Presentation 발표신청 |
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국가 |
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공동저자
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접수자
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Polycyclic aromatic hydrocarbons (PAHs) are a wide range of
environmental pollutants generated during the combustion of carbonaceous
materials at high temperatures. It is a highly toxic substance with various
side effects on human health when exposed. Therefore, a practical and sensitive
analytical method for examining PAH exposure in humans is required. Monitoring
human exposure to these requires observation of metabolites of biological
samples. However, the current hydroxylated polycyclic aromatic hydrocarbons
(OH-PAHs) analysis method using liquid chromatography-mass spectrometry
(LC-MS/MS) suffers from low ionization efficiency and isomer separation. In
particular, the problem of peak separation of many isomers must be optimized.
This study investigated the effects of mobile and stationary phase
composition on peak separation and chromatographic behavior. First, the
gradient program was optimized based on the ionization efficiency according to
the organic solvent content. Next, peak separation patterns were investigated
through various combinations of C18, AR, and pentafluorophenyl (PFP) columns as
stationary phases and no additive, ammonium acetate, and ammonium fluoride as
mobile phase additives. As a result, OH-NAP and OH-FLU were separated entirely,
and OH-PHE was partially separated to achieve good chromatographic behavior. These
results help optimize parameters such as mobile phase composition, column, and
gradient when developing an OH-PAHs analysis method.
This
study was supported by an intramural grant from Korea Institute of Science and
Technology.
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