여름정기학술대회
2022여름초록
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공동저자
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To analysis of oxysterols as oxidized cholesterol is important and is highly challenging due to
their low concentration in biological fluids. Thus, highly sensitive and selective analytical method
is essential for the simultaneous quantitative determination of the hydroxycholesterols (OHCs)
concentration. In this study, we developed analytical method to overcome the bottleneck via
picolinyl ester (PE) derivatization, and validated for six OHCs including 24S-OHC, 25-OHC, 27-
OHC, 24-diOHC, 25-diOHC, and 27-diOHCs in serum. Calibration curves were obtained over a
linear range of 0.1 to 250 ng/mL for oxysterols except 25-OHC and 0.5 to 250 ng/mL for 25-OHC.
Intra- and inter-day precisions were between 1.0% and 9.8% and the accuracies ranged from 87.6%
to 114.2%. Limit of detection and lower limit of quantification were 0.05 ng/mL and 0.1 ng/mL
for oxysterols except 25-OHC, and 0.1 ng/mL and 0.5 ng/mL for 25-OHC, respectively. Processed
sample stabilities and recoveries were obtained in range of 86.3% to 116.8% and 32.5% to 107.6%,
respectively. Pooled serums from obtained different batches were determined for oxysterol
analysis. Profiles of oxysterols in serum were successfully applied and evaluated. OHCs spiked
serums for potential biomarker discovery were preliminarily analyzed.
In conclusion, we presented a highly sensitive method for quantification of oxysterols in human
serum by LC-ESI/MS/MS with PE derivatization. This method is powerful tool and provides
reproducibility and reliability for simultaneous analysis of oxysterols.
* This research was supported by the Basic Science Research Program through the National
Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No.
2020R1F1A1071199).
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