여름정기학술대회
2022여름초록
발표자 및 발표 내용
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포스터발표 |
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Brief Oral Presentation 발표신청 |
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국가 |
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공동저자
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접수자
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According to the Korea Ministry of Health and Welfare, Korea's
fertility rate doubled decrease in 2017 compared to 2000. A miscarriage is a
spontaneous loss of pregnancy before the 20th gestational weeks, and it has
been reported that 10-20% miscarriage of the total pregnancies. Another
pregnancy related problem is a premature birth, which is a delivery of infants
before 37 weeks of pregnancy. There are various reports that the recent decline
in the fertility rate is closely related to miscarriage and premature birth.
Dansyl chloride(DNS-Cl) has been widely used as an efficient
fluorescent probe for the quantitative analysis of complex molecules with the
following functional groups, primary/secondary amines, phenolics, hydroxyl and
thiol groups. DNS-Cl has been used to induce and enhance the sensitivity of
urine metabolites in pregnant women. To discover potential metabolic biomarkers
in urine samples from patients with miscarriage and premature birth, the
relative amounts of metabolites in urine samples from normal pregnancies were
compared with those from patients with miscarriage and premature birth.
Derivatized metabolites were identified and quantified using
liquid chromatography tandem mass spectrometry (LC-MS/MS). Retention orders of
the derivatized metabolites were investigated from the databases, human
metabolome database (HMDB) and MyCompoundID. Six different standard metabolites
(L-Arginine, Glycine, L-Proline, L-Phenylalanine, L-lysine, Serotonin) were
used to set the relative retention orders of the metabolites in the
chromatographic separation. Another set of urinary metabolites were derivatized
with 13C-exchanged DNS-Cl and used as an internal standard. Relative
quantification of urine metabolites was performed by adding internal standards
to urine samples of various gestational weeks. After determining the relative
amounts of urinary metabolites, the amount of creatinine in each urine sample
was used for normalization.
As a result, three potential biomarkers (Ornithine,
Vanillylmandelic acid, and Hydroxyphenyllactic acid) were found in the volcano
plot, one of the statistical analysis. Further sample analysis will confirm the
consistency of the three potential biomarkers in the future.
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