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2022여름초록

제목

Liquid chromatography mass spectrometry analysis of urinary metabolites from miscarriage and premature birth

작성자
유원준

발표자 및 발표 내용

소속
서강대학교
발표구분
포스터발표
포스터발표
2. Mass Spectrometry in Elemental Analysis
Brief Oral Presentation 발표신청
신청자에 한함
Keyword
LC-MS/MS
metabolites
urine
miscarriage
premature birth

주저자

이름
유원준
소속
서강대학교
국가
대한민국

공동저자

공동저자
이름
오한빈
소속
서강대학교
국가
대한민국
이름
소속
국가
이름
소속
국가
이름
소속
국가
이름
소속
국가
이름
소속
국가
이름
소속
국가
이름
소속
국가
이름
소속
국가
이름
소속
국가

접수자

이름
유원준
소속
서강대학교

According to the Korea Ministry of Health and Welfare, Korea's fertility rate doubled decrease in 2017 compared to 2000. A miscarriage is a spontaneous loss of pregnancy before the 20th gestational weeks, and it has been reported that 10-20% miscarriage of the total pregnancies. Another pregnancy related problem is a premature birth, which is a delivery of infants before 37 weeks of pregnancy. There are various reports that the recent decline in the fertility rate is closely related to miscarriage and premature birth.

Dansyl chloride(DNS-Cl) has been widely used as an efficient fluorescent probe for the quantitative analysis of complex molecules with the following functional groups, primary/secondary amines, phenolics, hydroxyl and thiol groups. DNS-Cl has been used to induce and enhance the sensitivity of urine metabolites in pregnant women. To discover potential metabolic biomarkers in urine samples from patients with miscarriage and premature birth, the relative amounts of metabolites in urine samples from normal pregnancies were compared with those from patients with miscarriage and premature birth.

Derivatized metabolites were identified and quantified using liquid chromatography tandem mass spectrometry (LC-MS/MS). Retention orders of the derivatized metabolites were investigated from the databases, human metabolome database (HMDB) and MyCompoundID. Six different standard metabolites (L-Arginine, Glycine, L-Proline, L-Phenylalanine, L-lysine, Serotonin) were used to set the relative retention orders of the metabolites in the chromatographic separation. Another set of urinary metabolites were derivatized with 13C-exchanged DNS-Cl and used as an internal standard. Relative quantification of urine metabolites was performed by adding internal standards to urine samples of various gestational weeks. After determining the relative amounts of urinary metabolites, the amount of creatinine in each urine sample was used for normalization.

As a result, three potential biomarkers (Ornithine, Vanillylmandelic acid, and Hydroxyphenyllactic acid) were found in the volcano plot, one of the statistical analysis. Further sample analysis will confirm the consistency of the three potential biomarkers in the future.


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