The ministry of food and drug safety (MFDS) has set its pesticides residue level limits in livestock at 0.01 mg/kg and less through 2024, named as positive list system. Thus, it becomes necessary to establish a robust analytical method to determine the maximum residue limit of pesticides for these animals. Herein, we analyzed triazole fungicides, a known ergosterol biosynthesis inhibitor pesticides, commonly used in livestock feeds. We have developed a bioanalytical method to measure trace levels of triazole fungicides, including flutriafol, tebuconazole, and propiconazole in daily goats. Rats were used to construct a predictive toxicity model targeting translocation of pesticide residues in rat muscle, fat, and plasma. We applied the QuEChERS method to extract triazoles in daily goat and rat samples and then measured three triazole levels using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Our method provides reliable linearity (r²>0.99), accuracy (≤15% RE), and precision (≤15% CV). Recovery tests (≤15% CV) also demonstrate efficient extraction. Thus, our method has shown success at determining triazole fungicide residues in biological samples that is sufficient to meet MFDS standards.