Amphenicols are a group of chemical compounds in cluding chloramphenicol (CAP), thiamphenicol (TAP), florfenicol (FF), and florfenicol amine (FFA). In this work, a modified QuEChERS extraction method followed by UPLC-MS/MS was developed for the simultaneous quantification of residues, including CAP, TAP, FF, and FFA in egg and milk. The target analytes were extracted with a combination of acidified acetonitrile and ammonium hydorxide subsequently purified with original QuEChERS kits (composed of magnesium sulfate and sodium chloride) as well as n-hexane. All analytes were separated on a reversed-phase analytical column using and a mobile phase of (A) distilled water and (B) acetonitrile with 0.1% acetic acid. Good linearity (R²≥0.9958) was achieved over the tested concentration range (0.5–5 μg/kg for CAP, 5–50 μg/kg for TAP, FF, and FFA) in matrix-matched standard calibrations. Recoveries of 69.8~102.8%, with relative standard deviations (RSDs) of ≤8.87% (intraday and interday), were obtained for each sample at three spiking concentrations (CAP:0.5, 1, 5 μg/kg; TAP, FF, and FFA:5, 10, and 20 μg/kg). In conclusion, the current approach is simple, efficient and reliable and can successfully determine the residual levels of CAP, TAP, FF, and FFA in milk and egg. The study was supported by the Ministry of Food and Drug Safety Administration, Republic of Korea [grant number 20162MFDS621] in 2021.