겨울 심포지움
2018겨울초록
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포스터발표 |
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공동저자
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접수자
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Bloodstain is produced by the blood left at the crime scene, and it contains a lot of information. The elapsed time since the bloodstain was formed is called the age of a bloodstain, which is very important because it can identify the time of the attack. Although various methods have been reported to confirm the elapsed time of bloodstain, it has not yet been applied to the forensic analysis. Therefore, we used a mass spectrometer to analyze the metabolite of bloodstain to identify the new age of bloodstain markers. In this study, blood samples were collected from 6 healthy volunteers and bloodstain was prepared using filter paper. Metabolites were extracted from 0, 7, 14, and 21 days after collection and analyzed using HPLC Q-TOF. An average of 580 (range from 378 to 857) molecular features was identified in mass spectrometry (MS) data, and an average of 388 (range from 256 to 537) molecular features was identified in tandem mass spectrometry (MS/MS) data. In addition, it was confirmed that metabolites significantly increased or decreased at 7 days, 14 days, and 21 days were compared to day 0. Multivariate statistical data analysis (PCA, PLS-DA, and OPLS-DA) was performed to find candidates for the age of a bloodstain. The candidates selected through statistical analysis were identified by MSMS spectrum matching of METLIN database. We have found that changed metabolites according to elapsed time and the bloodstain as time passed can be classified with them. Therefore, it will be possible to estimate the elapsed time of the blood which is obtained at the scene of the incident.
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