Environment factors are significantly correlated with manifestations of pathogenesis. Profile of proteins of beagle organ tissue by mass spectrometry (MS) is necessary to early diagnosis of human disease by companion animal. Through proteomic analysis, we found 10,498 protein groups and 16,273 proteins in the database that has 28,946 proteins in Canis lupus familiaris of species. Based on this result, the identified protein information enables to utilize as beagle proteome library. In statistical analysis, the result was found 143 tissue enriched proteins in 12 organ tissue samples. We suggest that these tissue enriched proteins could be biomarkers of organ specific disease. Notably, for each organ tissue, number of identified protein groups was more than 3000 and the total proteins were covered in 56.22% of beagle protein database. In addition to the beagle protein library, major benefits are now being made in companion medicine spilling over into developments for human healthcare.