겨울 심포지움
2018겨울초록
발표자 및 발표 내용
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주저자
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공동저자
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접수자
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The collection of patient whole blood on paper, known as dried blood spot (DBS), was introduced in 1963 for the screening of inherited metabolic diseases in neonates. Compared with conventional venous blood sampling, DBS sampling is less invasive method, and easy to keep and transport samples. In this study, an isotope dilution-ultra performance liquid chromatography–tandem mass spectrometry (ID-LC-MS/MS) was investigated to quantify glycated hemoglobin (HbA1c) in DBS. HbA1c has been widely used to assess glycemic control in diabetes mellitus. We used only one disc of 3.2-mm diameter for quantification of HbA1c, and that is about 3 µL of whole blood.
The bias factor caused by underestimation of area was strictly investigated and revised with area calculation software. The results were compared with areas by manual technique using ruler, or nominal value of puncher. The accuracy and precision of measurement showed sufficient results in the optimized sample treatment conditions. The variations of different position within a spot showed acceptable results (<3 %). As some unstable compounds in blood are easily susceptible to harsh environmental conditions, we examined the short-term effect of temperature (1-50 oC) and humidity (<30% and 30- 70%) during a period of 10 days on the sample stability. The optimized analytical method was applied to real blood samples, and results showed excellent comparability with those of venous methods. This study provides reliability to constitute a practical procedure for a rapid measurement of HbA1c.
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