2026년
한국질량분석학회 여름학술대회 및 총회
2026. 08.19 (수) ~ 2026. 08.21 (금)
창원컨벤션센터(CECO)
초록검색
| 제목 | Bronchoalveolar lavage proteomics in exacerbation of bronchiectasis |
|---|---|
| 작성자 | 이주연 (한국기초과학지원연구원) |
| 발표구분 | 포스터발표 |
| 발표분야 | 6. General |
| 발표자 |
Ju Yeon Lee (Korea Basic Science Institute) |
| 주저자 | Ju Yeon Lee (Korea Basic Science Institute) |
| 교신저자 |
Bumhee Yang (Chungbuk National University College of Medicine) |
| 저자 |
Ju Yeon Lee (Korea Basic Science Institute) Jiyoul Yang (Chungbuk National University College of Medicine) Jin Young Kim (Korea Basic Science Institute) Yeji Do (DGIST) Min-Sik Kim (DGIST) Dong Eun Kye (Chungbuk National University College of Medicine) Geonhui Min (Chungbuk National University) In-Sook Jeon (Chungbuk National University College of Medicine) Eung-Gook Kim (Chungbuk National University College of Medicine) Joong Kook Choi (Chungbuk National University College of Medicine) Minjae Choi (Chungbuk National University Hospital) Hyun Lee (Hanyang University College of Medicine) Bumhee Yang (Chungbuk National University College of Medicine) |
|
The molecular basis of bronchiectasis exacerbation remains poorly characterized at the proteomic level. We profiled bronchoalveolar lavage (BAL) fluid from patients in exacerbation (n = 4) and stable (n = 4) states using TMTpro 16-plex labeling, high-pH fractionation, and nanoLC-MS/MS on an Orbitrap Fusion Lumos (DDA mode). Proteins were identified via IP2/ProLuCID (1% FDR) and quantified by summed TMT reporter intensities. A
total of 1,577 proteins were identified with high reproducibility (Pearson r
> 0.9). Of these, 127 proteins differed significantly between groups (p <
0.05), including 23 with >2-fold changes: 18 upregulated during exacerbation
(CRP, S100A8, S100A9, BPI, ORM1) and 5 downregulated mucosal/immune defense
proteins (MUC5B, MUC5AC, IGHA1, HSPE1, KLK13). PCA and hierarchical clustering
clearly separated exacerbation from stable samples. Pathway enrichment
identified neutrophil degranulation as the top activated pathway, followed by
C-type lectin receptor signaling and innate immune response. |
|