2026년
한국질량분석학회 여름학술대회 및 총회
2026. 08.19 (수) ~ 2026. 08.21 (금)
창원컨벤션센터(CECO)
초록검색
| 제목 | Integrated LC-MS Characterization of Glycosylation in Trastuzumab and Antibody-Drug Conjugate Standards |
|---|---|
| 작성자 | 서문성인 (성신여자대학교) |
| 발표구분 | 포스터발표 |
| 발표분야 | 2. Mass Spectrometry in Elemental Analysis |
| 발표자 |
서문성인 (성신여자대학교) |
| 주저자 | 서문성인 (성신여자대학교) |
| 교신저자 |
고병준 (성신여자대학교) |
| 저자 |
서문성인 (성신여자대학교) 고병준 (성신여자대학교) |
|
In antibody–drug conjugates (ADCs), Fc N-glycosylation is a critical quality attribute, and comparing glycan profiles across products offers useful insight for characterization. We established an LC–MS workflow to identify and compare the Fc N-glycans of trastuzumab (Herceptin) and four ADC standards (Kadcyla, Enhertu, Trodelvy, Adcetris). Released N-glycan profiling (RapiFluor-MS, HILIC-UPLC-FLR) assigned major glycans by retention time, and TCEP-reduced heavy-chain intact mass gave orthogonal confirmation through the characteristic +162 Da (galactose) and −146 Da (fucose) spacing. Both methods identified a G0F-dominant, fucosylated biantennary set (G0/G0F/G1F/G2F). Herceptin and Kadcyla were nearly identical, whereas Enhertu—despite the shared trastuzumab backbone—was markedly enriched in G0F, and Trodelvy and Adcetris showed product-specific distributions. Pairing released-glycan and intact-mass analysis provides a robust orthogonal approach for Fc N-glycan identification in ADC standards. |
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