2025년
한국질량분석학회 여름학술대회 및 총회
2025. 08.27 (수) ~ 2025. 08.29 (금)
부산항국제전시컨벤션센터(BPEX)
초록검색
| 제목 | Method development of amine-containing metabolite analysis using di-tert-butyl decarbonate derivatization coupled with liquid chromatography-mass spectrometry |
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| 작성자 | 최용수 (한국과학기술연구원) |
| 발표구분 | 포스터발표 |
| 발표분야 | 6. General |
| 발표자 |
최용수 (한국과학기술연구원 천연물 연구소) |
| 주저자 | 최용수 (한국과학기술연구원 천연물 연구소) |
| 교신저자 |
최용수 (한국과학기술연구원 천연물 연구소) |
| 저자 |
최용수 (한국과학기술연구원 천연물 연구소) |
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Amine metabolites play crucial roles in biological systems and serve as biomarkers for various disease. In this study, we developed a cost-effective derivatization method based on N-tert-butyloxycarbonylation (Boc), which is the most affordable among previously reported amine derivatization methods. This enviromnetally friendly approach enhances the retention and detection of amine metabolites in reversed-phase liquid chromatography-mass spectrometry, with Boc dedrivatives demonstring excellent stability for at least 20 days at 4 oC and 60 days at -20 oC. Importantly, Boc derivatization does not affect the signal intensity or the number of non-amine metabolites, ensuring the method’s compatibility with untargeted metabolomics workflows. The effectiveness of this method was validated using immature rice seed extracts from Japnica and Indica cultivars. Boc derivatization enabled the signal detection of 54 amine metabolites, with comparative analysis identifying 11 significantly differentiated metabolites between the reice varieties, including histidine, methionine, pipecolate, and GABA. The specificity and robustness of this method confirm its utility for discovering biologically relevant metabolites in complex biological and agricultural samples |
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