2025년
한국질량분석학회 여름학술대회 및 총회
2025. 08.27 (수) ~ 2025. 08.29 (금)
부산항국제전시컨벤션센터(BPEX)
초록검색
| 제목 | Multi-omics-based elucidation of catechol-induced oxidative and neurodevelopmental toxicity via proteomic and signaling network analysis in zebrafish |
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| 작성자 | 정희정 (한국기초과학지원연구원) |
| 발표구분 | 포스터발표 |
| 발표분야 | 3. Food & Environment |
| 발표자 |
Hee-Chung Chung (Digital Omics Research Center, Korea Basic Science Institute) |
| 주저자 | Puspanjali Swain (Department of Biology, Chungnam National University) |
| 교신저자 |
Joseph Kwon (Digital Omics Research Center, Korea Basic Science Institute) Cheol-Hee-Kim (Department of Biology, Chungnam National University) |
| 저자 |
Puspanjali Swain (Department of Biology, Chungnam National University) Hee-Chung Chung (Digital Omics Research Center, Korea Basic Science Institute) Tae-Yoon Kim (Department of Biology, Chungnam National University) Kang-Han Lee (Department of Biology, Chungnam National University) In-Geol Choi (Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University) Sang Hee Lee (Risk Assessment Division, National Institute of Environmental Research, Ministry of Environment) Woo-Keun Kim (Center for Predictive Model Research, Korea Institute of Toxicology) Joseph Kwon (Digital Omics Research Center, Korea Basic Science Institute) Cheol-Hee-Kim (Department of Biology, Chungnam National University) |
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Catechol (1,2-dihydroxybenzene) an environmental neurotoxicant, disrupts vertebrate development by altering cellular redox status and signaling balance. Our study focused on quantitative proteomic analysis in zebrafish embryos at 48 hpf, integrating LC-MS/MS-based quantitative proteomics and IPA-based pathway modeling. Proteomic profiling identified significant modulation of calcium transporters (Atp2a1/2), cytoskeletal proteins (Tubb2b, Myh4), and redox effectors (Gstp1, Pdia3), indicating concurrent ionic imbalance and oxidative stress. Network analysis using IPA revealed six1-dependent repression of the neurotrophic factor ntf3, potentially compromising neurogenesis and neuronal maintenance. This transcriptional suppression coincides with downregulation of Igf1–Raf1 and Pi3k–Akt signaling, key pathways in neuronal survival. Spatial expression confirmed gstp1 and ca2 enrichment in ionocytes and neuronal cells . Our findings emphasize that catechol-induced developmental toxicity is mediated through transcriptional regulation of neurotrophic signaling, captured effectively through proteomic perturbation mapping. This study underscores the importance of proteome-informed toxicological assessments in early developmental models. |
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