2025년

한국질량분석학회 여름학술대회 및 총회

2025. 08.27 (수) ~ 2025. 08.29 (금)

부산항국제전시컨벤션센터(BPEX)

초록검색

  |  한국질량분석학회 여름학술대회 및 총회 Brief Oral Presentaionof Selected Posters
제목 Targeted Metabolomic Profiling of Plasma and Brain Tissues in a Lipopolysaccharide-induced Neuroinflammation Mouse Model Using LC-MS/MS
작성자 이경아 (충남대학교)
발표구분 포스터발표
발표분야 6. General
발표자

이경아 (한국화학연구원)
주저자 이경아 (한국화학연구원)
교신저자

김상겸 (충남대학교 약학대학)

배명애 (한국화학연구원)
저자

이경아 (한국화학연구원)

정우진 (한국화학연구원)

김성순 (한국화학연구원)

양정윤 (한국화학연구원)

이병회 (한국화학연구원)

신대섭 (한국화학연구원)

황규석 (한국화학연구원)

이성민 (한국화학연구원)

김가현 (한국화학연구원)

김상겸 (충남대학교 약학대학)

배명애 (한국화학연구원)

Neuroinflammation plays a critical role in the pathogenesis of various neurodegenerative and psychiatric disorders. In this study, we employed a mouse model of neuroinflammation induced by systemic administration of lipopolysaccharide (LPS, 3 or 10 mg/kg, i.p.) to investigate metabolomic changes. Plasma and brain samples were collected at 4 hours after LPS injection. A broad range of amino acid-related neurotransmitters including histamine, acetylcholine, dopamine, serotonin, norepinephrine, γ-aminobutyric acid, and glutamic acid were quantitatively analyzed using ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). LPS administration resulted in a significant decrease in dopaminergic pathway metabolites including dopamine, norepinephrine, homovanillic acid levels in plasma and brain while glutamic acid and 3-hydroxykynurenine levels were significantly increased in brain tissue, suggesting excitotoxicity and neurotransmitter imbalance. These findings indicate that LPS-induced neuroinflammation leads to dysregulation of neurotransmitter synthetic pathways in both peripheral and central nervous systems. This integrative approach combining targeted metabolomic profiling provides insight into the biochemical landscape of acute neuroinflammation and may support the discovery of potential biomarkers for neuroimmune dysregulation.
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