안지호 ( Korea International School (KIS))

강지우 (채드윅 송도 국제학교)

정의길 (네이코)

정진아 (Wesleyan School)

안지호 ( Korea International School (KIS))

강지우 (채드윅 송도 국제학교)

정의길 (네이코)

Alzheimer’s disease (AD), a progressive neurodegenerative disorder, is the most common type of dementia that impairs one’s cognitive and memory functions. Despite scientists’ ongoing efforts, no definite cure has been discovered yet. This study aims to contribute to an effective treatment for AD. The differences and effects of  5xFAD (transgenic model of familial AD) mouse and Wild-type (non-modified control) mouse (WT) were analyzed. Hippocampal tissue was sectioned via cryostat, mounted on ITO-glass, and coated using HTX-M5 to preserve molecular integrity and enable ionization. Fourier Transform Ion Cyclotron Resonance (FT-ICR MS) was applied to identify and visualize molecular differences between 5xFAD and WT samples. Lipid intensity data were gathered for both 5xFAD and WT. Lipids of inflammatory and pathological modifications (SHexCer, Cep, Hex2Cer, and MIPC) were commonly found in 5xFAD. Lipids of the neurotic defense alignment (CerPE, SM, HexCer, and IPC) were found in masses throughout the WT model, which stipulates high stabilization and maintenance of synapse functions. Through this method, the dispersed AD molecules across the brain were observed. Emphasis was placed on the usage of high-resolution imaging mass spectrometry with mouse models to identify the molecular changes under the influence of AD. This study offers potential insights that contribute to future innovations in treatment and diagnosis research.