임유진 (성균관대학교 약학과)

임유진 (성균관대학교 약학과)

안지아 (성균관대학교 약학과)

황윤아 (성균관대학교 약학과)

강민식 (한국과학기술연구원 도핑콘트롤센터)

이재익 (한국과학기술연구원 도핑콘트롤센터)

이상규 (성균관대학교 약학과)

Nutritional status is a regulator of metabolic homeostasis, and fluctuations in nutrient supply can have a profound effect on physiological functions. While prolonged deprivation is harmful, controlled restriction−such as intermittent fasting−has been linked to lifespan extension and delayed age-related diseases. Starvation is a form of acute and extreme nutritional stress that induces systemic metabolic adaptations, in which the liver plays a central role. In this study, we performed proteometabolomics based on mass spectrometry to investigate liver-specific molecular changes in response to starvation. Male mice were starved for 48 h, and liver tissues were analyzed. A total of 5,632 proteins and 259 metabolites were quantified, of which 270 proteins and 153 metabolites were significantly altered. The integrated omics analysis revealed major pathway alterations such as lipid metabolism (e.g., glycerophospholipids), energy metabolism (TCA cycle, glycolysis/gluconeogenesis), amino acid and antioxidant systems (cysteine and methionine metabolism), and sugar mobilization (starch and sucrose metabolism). These changes reflect energy balance, oxidative stress response, and structural remodeling as adaptive responses to starvation. The findings of this study offer insights for understanding the metabolic adaptation mechanisms of the liver during acute starvation and may aid in the developing of novel therapeutic strategies for the management of metabolic diseases.