2025년
한국질량분석학회 여름학술대회 및 총회
2025. 08.27 (수) ~ 2025. 08.29 (금)
부산항국제전시컨벤션센터(BPEX)
초록검색
| 제목 | Glycan Profiling Analysis of Therapeutic Antibodies |
|---|---|
| 작성자 | 이재범 (성신여자대학교) |
| 발표구분 | 포스터발표 |
| 발표분야 | 2. Mass Spectrometry in Elemental Analysis |
| 발표자 |
이재범 (성신여자대학교) |
| 주저자 | 이재범 (성신여자대학교) |
| 교신저자 |
고병준 (성신여자대학교) |
| 저자 |
이재범 (성신여자대학교) 고병준 (성신여자대학교) 서문성인 (성신여자대학교) |
|
Glycosylation is a key factor influencing the efficacy, stability, and immunogenicity of therapeutic antibodies. Typically, when the combined proportion of G0F, G1F, and G2F exceeds 90%, the antibody is considered to have favorable tertiary structural stability and an extended in vivo half-life. In this study, we conducted glycan profiling to evaluate the glycosylation patterns of several antibody samples. The analysis was carried out using ultra-high-performance liquid chromatography (UHPLC) coupled with mass spectrometry to identify critical glycan structures, such as fucosylated forms and high-mannose variants. Our findings showed highly reproducible glycan profiles across triplicate experiments, highlighting the robustness and reliability of the analytical platform for consistent glycan characterization. |
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