2025년
한국질량분석학회 여름학술대회 및 총회
2025. 08.27 (수) ~ 2025. 08.29 (금)
부산항국제전시컨벤션센터(BPEX)
초록검색
| | 한국질량분석학회 여름학술대회 및 총회 Brief Oral Presentaionof Selected Posters | |
| 제목 | Comprehensive Gas-Phase Characterization of Therapeutic Antibodies: Native MS and CIU Reveal Subclass-Specific Conformational Landscapes |
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| 작성자 | 모줌다르 아비크 (University of Science and Technology) |
| 발표구분 | 포스터발표 |
| 발표분야 | 4. Medical / Pharmaceutical Science |
| 발표자 |
Abhik Mojumdar (Korea Basic Science Institute) |
| 주저자 | Abhik Mojumdar (Korea Basic Science Institute) |
| 교신저자 |
Kun Cho (Korea Basic Science Institute) |
| 저자 |
Abhik Mojumdar (Korea Basic Science Institute) Heejin Yoo (Korea Basic Science Institute) Minsun Kim (Korea Basic Science Institute) Sunhee Choi (Korea Basic Science Institute) Kun Cho (Korea Basic Science Institute) |
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Understanding the structural integrity and conformational stability of monoclonal antibodies (mAbs) is critical for therapeutic development. In this study, we apply a combined native mass spectrometry (MS) and cyclic ion mobility spectrometry (cIMS) platform to characterize IgG subclass antibodies. Using size-exclusion chromatography coupled with native MS and collision-induced unfolding (CIU), we compare intact mass profiles, glycosylation patterns, and gas-phase stability across IgG1, IgG3, and IgG4 standards. CIU fingerprinting reveals distinct subclass-dependent unfolding behaviors, while enzymatic deglycosylation demonstrates the structural role of Fc glycans. Benchmarking therapeutic mAbs such as Adalimumab and Pembrolizumab further highlights subclass-specific conformational traits. This integrated approach enables high-resolution differentiation of mAb structural variants, offering valuable insights into stability, formulation quality, and subclass functionality. Our findings demonstrate the utility of CIU and cIMS-MS for comprehensive mAb characterization in both research and biopharmaceutical quality control settings. |
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