장귀주 (가천대학교)

한상윤 (가천대학교)

장귀주 (가천대학교)

임고은 (가천대학교)

공태일 (가천대학교)

한상윤 (가천대학교)

The cellular uptake of nanoparticles (NPs) is influenced not only by their functional design but also by the biological molecules they acquire in biological fluids. These adsorbed biomolecules, known as biomolecular coronas, form a dynamic interface that governs NP–cell interactions. Circulating lipids play essential roles in transport, membrane structure, and signaling, of which coronas on NPs are increasingly recognized for their potential impact on NP behavior in vivo. In this study, we investigate the influence of the lipid coronas formed from HDL, LDL, and serum on the cellular uptake of liposomal drugs. To assess the influence, cationic liposomes were incubated with HDL, LDL, and whole serum to form their coronas around them. The corona compositions were analyzed using bioassays, nanoparticle tracking analysis, and untargeted LC-MS/MS. In the results, distinct differences were observed between the lipid composition in the respective incubation fluids and that in the resulting hard coronas, revealing preferential enrichment of particular lipids on the liposomal surfaces. Cellular uptake experiments revealed that the extent of liposome internalization varied depending on the employed biological fluids, suggesting that the composition of lipid coronas may influence NP–cell interactions. This study would deepen our understanding of how the biological milieu shapes nanocarrier behavior for improvement of the efficacy of nanomedicine systems.​