Chung-Oui, Hong (Animal and Plant Quarantine Agency)

Mi-Young, Park (Animal and Plant Quarantine Agency)

Chung-Oui, Hong (Animal and Plant Quarantine Agency)

Sunjin, Park (Animal and Plant Quarantine Agency)

Se-Hyung, Kim (Animal and Plant Quarantine Agency)

Seon-Young, Lee (Animal and Plant Quarantine Agency)

Inhae, Jeon (Animal and Plant Quarantine Agency)

Hyun-Ok, Ku (Animal and Plant Quarantine Agency)

Mi-Young, Park (Animal and Plant Quarantine Agency)

Dinotefuran, a systemic neonicotinoid insecticide, has been widely used for the control a range of sucking insects, including during Korea’s nationwide response to the first outbreak of Lumpy skin disease(LSD) in cattle in October 2023. Given its environmental applications to suppress insect vectors such as mosquitoes and flies, concerns over residual dinotefuran in edible animal tissues prompted regulatory interest in food safety and compliance. To assess potential dinotefuran contamination in cattle following its authorized use as a livestock facility insecticide, the Animal and Plant Quarantine Agency conducted an exploratory surveillance study in 2024 using 101 bovine samples collected nationwide. An LC-MS/MS method for dinotefuran detection in bovine muscle was validated in accordance with Codex Guidelines and the Korean Food Code. The method demonstrated a limit of quantification(LOQ) of 5.0 μg/kg, high accuracies within 3.69% and coefficients of variation(%CV) of 3.62% and 5.18% for intraday and interday precision, respectively. No dinotefuran residues were detected in any of the 101 muscle samples, suggesting proper field application of dinotefuran during LSD control. In Korea, the maximum residue limit(MRL) for dinotefuran in livestock products has been set at 0.01 mg/kg according to positive list systrem. The results of this study confirm that dinotefuran usage in Korea complies with both domestic regulatory standards and international food safety assessments.