2025년
한국질량분석학회 여름학술대회 및 총회
2025. 08.27 (수) ~ 2025. 08.29 (금)
부산항국제전시컨벤션센터(BPEX)
초록검색
| 제목 | LC-MS/MS profiling and molecular networking of glucosinolates induced in broccoli sprouts by a biotechnological tool |
|---|---|
| 작성자 | 최하늘 (충남대학교) |
| 발표구분 | 포스터발표 |
| 발표분야 | 3. Food & Environment |
| 발표자 |
최하늘 (충남대학교) |
| 주저자 | 최하늘 (충남대학교) |
| 교신저자 |
박해민 (충남대학교) |
| 저자 |
최하늘 (충남대학교) 박해민 (충남대학교) |
|
Glucosinolates (GSLs) are health-promoting secondary metabolites predominantly found in broccoli. In this study, a total of 12 GSLs were identified using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS), and three major GSLs-glucoraphanin (GRA), glucobrassicin (GBS), and neoglucobrassicin (NGBS)-were selected for quantitative analysis. To enhance their accumulation, we utilized a biotechnological tool based on regulatory insights into GSL biosynthesis, which resulted in the promotion of both aliphatic and indole GSL pathways by upregulating related biosynthetic genes. Additionally, molecular networking analysis coupled with RDBE-based structure prediction facilitated the identification of two new GSLs. Thus, the enhanced accumulation of main bioactive GSLs and the identification of new potential candidate GSLs in broccoli sprouts are anticipated to support nutraceutical research. |
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