Perfluorooctanoic acid (PFOA) are widely used in various industrial and commercial applications including polishes, food packaging, surfactants, fabrics, and coatings for paper. Due to the strong stability to metabolic and environmental degradation, the persistence nature and potential toxicity of these substances have raised substantial concerns about health effects.
In this study, we have confirm and investigated the gender and age differences in pharmacokinetic (PK) characteristics and tissue distribution of PFOA. For this study, a simultaneous determination method of the PFOA in rat plasma, tissues, urine, feces was developed and validated using a bio-liquid chromatography triple quadrupole mass spectrometry (Bio LC/TQ).
PFOA in plasma, tissues, urine, and feces matrix showed an excellent linear relationship in the range of 0.2~50,000 ng/mL, with linear regression coefficients reaching 0.996~0.999. The lower limit of quantification (LLOQ) for PFOA were at 39.1~ 781 ng/mL levels in plasma, tissues, urine, and feces matrix. The method was also accurate and precise, with spiking recoveries in all tested matrices ranging from 81.72 to 114.15 %, with RSDs within 0.40~4.20%. Our resuts suggests that the method can reliably be applied for the routine measurement of trace PFOA in various biological sample. We are now analyzing the PK parameters in plasma, tissues, urine, and feces samples and predicting the Kp value of plasma, tissues, urine, and feces.