여름정기학술대회
2022여름초록
발표자 및 발표 내용
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발표구분 |
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포스터발표 |
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Brief Oral Presentation 발표신청 |
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국가 |
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공동저자
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접수자
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Amyloid-Beta (Aβ) peptides
in human Plasma containing Aβ42 (residues 1–42) and Aβ40 (residues1–40), are utilized as biomarkers in work for the
diagnosis of Alzheimer’s disease. Aβ42 (residues 1–42) and Aβ40 (residues1–40) was determined in human plasma by LC-MS/MS using
isotopic as an internal standard. To simplification of extraction steps and
optimization of chromatographic condition, sample preparation method was
accomplished using solid phase extraction. The LC-MS/MS system was equipped
with an electrospray source operating in the positive ion mode. The lower limit
of quantification (LLOQ) was 20 pg/mL, using a sample volume of 200 μL for the
analysis. The reproducibility of the method was evaluated by analyzing five
replicates at four quality control (QC) levels over the nominal concentration
range Aβ42 and Aβ40, 20 to 500 pg/mL.
The
validation, reproducibility, stability, and recovery of the method were
evaluated. The method has been successfully applied to pharmacokinetic studies
of Aβ42 and Aβ40 in human plasma.
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