여름정기학술대회
2022여름초록
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Exploring Brain Cell-Specific N-glycome Diversity by LC-MS/MS towards Understanding Neural Network
- 작성자
- YINDONGTAN
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Brain cells contain various neuronal subtypes, with specific functions depending on their diversity. Although research on the brain has been ongoing for a long time, the specific functions of brain cells and the related molecular mechanisms have not been clearly clarified due to the diversity of cells. Glycosylation is one of the most common post-translational modifications (PTM) of proteins, reacting first in cell-to-cell interactions. Glycans are emerging as new molecular targets for cellular diversity studies due to their sensitively changing characteristics to environmental conditions. In this study, glycome expression was investigated using LC-MS/MS in neurons and glial cells, two major cell types containing the neural parenchyma. Interestingly, we observed cell-specific differences in glycome expression in neurons and glial cells when compared to the well-known brain tissue glycomes. Abundant N-glycomes in synaptosomes show a similar expression pattern of brain tissue glycosylation. These results may suggest that synaptosome glycosylation is highly related to glycosylation expressed in brain tissues. On the other hand, the abundance of glial cells was high in sialylated hybrid type glycans, which was significantly different from neurons and brain tissue. Sialic acid is a key factor in neuronal development and maintenance of neuronal plasticity. Thus, change in sialylation may explain the role of glial cells, providing a development environment for nerve cells. Finally, our findings showing differences in glycan types in representative brain cells suggest the need for cell-specific exploration to understand the brain function and molecular mechanisms of neural networks.
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