여름정기학술대회
2022여름초록
발표자 및 발표 내용
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공동저자
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Veterinary
drugs are used for animal therapeutic and prophylactic purposes to control
bacterial infections and prevent outbreaks of animal disease. When used
properly, the levels of veterinary drug residues in foods are usually very low,
so acute food poisoning incidents would not have occurred. However, since
veterinary drugs remaining in livestock products may be harmful to humans by
consuming them, it is necessary to develop a method for analyzing veterinary
drugs residues in foods. Antibiotics, especially those used in livestock,
increase the risk of developing antibiotic-resistant bacteria, and people who
ingest them may cause antibiotic-resistant infections. Vancomycin is an
antibiotic to treat serious infections caused by gram-positive bacteria, and in
Korea, it is regulated as a substance that should not be detected in food
(prohibited drugs). In this study, liquid chromatography-tandem mass
spectrometry was developed to analyze vancomycin, one of the banned substances
in pork, beef, chicken, shrimp, flatfish, eel, milk, and egg. Vancomycin is
easily decomposed in a strong base or acid within a short time. Considering
these characteristics, after extracting livestock and fishery products with a
15% acetonitrile solution containing 50 mM ammonium acetate, MCX SPE purification
method using water-saturated hexane was optimized. In this way, the recovery
rate was improved from less than 50% in the existing method to more than 80%,
and the limit of quantitation (LOQ) of 0.5 ppb was satisfactorily obtained. The
analytical method was validated according to the Codex guidelines
(CAC/GL-71-2009), and vancomycin was satisfied with selectivity, linearity,
recovery, accuracy, and precision in all matrices. This research was supported
by a grant (20162수산물622) from Ministry of Food and
Drug Safety in 2021.
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