여름정기학술대회
2022여름초록
발표자 및 발표 내용
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| Brief Oral Presentation 발표신청 |
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주저자
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공동저자
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접수자
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Found in all living organisms,
carbohydrates are the most abundant group of compounds in nature. Also called glycans,
they play a key role in regulating biological systems, such as protein folding,
trafficking, and cell-cell interactions. However, glycan analysis is very
challenging compared to other biomolecular assays due to its structural
complexity and diverse heterogeneity. In particular, limited standard products
and numerous isomers make quantitative analysis more difficult. Herein, we
developed a simultaneous analysis platform for characterization based on
LC/MRM-MS using 12 monosaccharides (mannose, glucose, galactose, fucose,
rhamnose, N-acetylglucosamine, N-acetylgalactosamine, ribose, xylose,
arabinose, glucuronic acid and galacturonic acid) commonly found in nature as a
novel strategy to quantify glycans through absolute monosaccharide quantitative
analysis. It is still analytically difficult to selectively separate and detect
monosaccharides of similar structures that exist in a wide range of
concentrations in samples containing complex matrices. Therefore, we introduced
a systematic method that combines complete baseline LC separation and
monitoring of structure-specific cross-ring cleavage ions of PMP-labeled
monosaccharides. Indeed, 12 monosaccharides could be successfully absolute
quantified from attomoles to femtomoles level in food and biopharmaceuticals. In
the future, our platform will be utilized to provide absolute quantitative
information on the glycan level through comprehensive interpretation of
monosaccharide and glycan analysis results.
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