여름정기학술대회
2022여름초록
발표자 및 발표 내용
| 소속 |
|
|---|---|
| 발표구분 |
|
| 포스터발표 |
|
| Brief Oral Presentation 발표신청 |
|
| Keyword |
|
주저자
| 이름 |
|
|---|---|
| 소속 |
|
| 국가 |
|
공동저자
| 공동저자 |
|
|---|
접수자
| 이름 |
|
|---|---|
| 소속 |
|
Ischemic stroke is one of the major causes of death and permanent disability in the world. Transient middle cerebral artery occlusion (MCAO) animal model in rats has been widely used for the efficacy studies for stroke. Blood-brain barrier (BBB) permeability is one of the indicators of measurement the extent of neurological injury in the MCAO. The most popular technique for measuring extent of BBB breakdown is a spectrometry technique using Evans-blue staining. However, there are limitations to quantifying the extent of BBB disruption. The concentrations of the drugs that penetrate brain cannot be quantified and is still unknown. Therefore, in this study, as an alternative method of quantification of test materials, mass spectrometry using liquid chromatography–mass spectrometry (LC-MS/MS) and desorption electrospray ionization (DESI)-mass spectrometry imaging (MSI) was used to measure the extent of BBB permeability of acetaminophen in a rat MCAO model with intraluminal suture surgery. The drug concentration in brain tissue was quantified by LC-MS/MS and total brain-to-plasma concentration ratio was calculated. DESI-MSI were able to visualize drug distribution in the ischemic hemisphere of the brain. These quantifying methods were available to obtain accurate and statistically reliable experimental results for the evaluation of drug in a rat MCAO model.
게시물수정
게시물 수정을 위해 비밀번호를 입력해주세요.
댓글삭제게시물삭제
게시물 삭제를 위해 비밀번호를 입력해주세요.