The efficacy of herbal medicines varies greatly depending on the conditions such as the climate, production area, collection time, origin, and contents of ingredients where they are produced. Therefore, it is possible to achieve scientification, modernization, and globalization of herbal medicines through accurate quality control and maintaining the same production conditions so that these various variables can be minimized, and the efficacy can be constant. As part of this quality standardization project, in this study, the separation and identification of volatile compounds in herbal medicine extracts were performed. Headspace gas chromatography-tandem mass spectrometry (HS-GC-MS/MS) with solid phase microextraction (SPME) is developed for the fast analysis of volatile constituents in the herbal medicines. The SPME fiber was PDMS, PDMS-DVB, and DVB-CAR-PDMS were investigated, and the best extraction was achieved with the mixed fiber DVB-CAR-PDMS. Several HS-SPME parameters, including fiber coating, extraction temperature, and extraction time, are optimized. Eighty-nine compounds in twenty types of herbal medicines were identified by HS-SPME-GC-MS/MS using the GC NIST MS library (Version. 2.3). When compared to the results of HS-GC-MS/MS, SPME method dramatically improves peak sensitivity and peak sharpness, providing good selectivity to identify volatile compounds in complicated matrices. In conclusion, HS-SPME is an effective analysis method for determining the volatile compounds present in the herbal medicines. This research was supported by a grant (20173첨단약561) from Ministry of Food and Drug Safety in 2021.