Ischemic stroke is classified into several subgroups based on causes. Large artery atherosclerosis is known as a major cause of ischemic stroke. Many studies have been reported that large artery atherosclerosis is closely associated with inflammation and several inflammation markers were also increased in patients with large artery atherosclerosis. However, there is no exact diagnostic marker for large artery atherosclerostic stroke. Most of studies have analyzed to identify novel biomarker in various disease using pooled serum samples. However, these studies can lead to biased results. Our aim is to analyze individual serum sample instead of pooled serum sample. 52 patients and 43 healthy subjects are used for biomarker of large artery atherosclerosis stroke. Quantification of identified 514 serum proteins were performed by SWATH-MS acquisition. As a result, differentially expressed proteins by more than 2 fold were 149. The result of functional analysis showed that the differentially expressed proteins are associated with alternative complement pathway-related immune response and positive regulation of tolerance induction to nonself antigen. The comparative analysis of serum proteins in patients with large artery atherosclerosis stroke and healthy subjects helps to understand pathophysiology of large artery atherosclerosis. We expect that inflammation-related proteins will distinguish subtype of stroke from healthy subjects.