In this study, a simple and rapid lipid extraction method is introduced. The effective lipid extraction from biological systems with a minimization of the matrix effect is important for the successful qualitative and quantitative analysis of lipids using mass spectrometry. We have devised the simple, rapid, and efficient lipid extraction method using super absorbent polymers (SAPs) for the purpose of isolating lipids from biological fluids. The SAPs method conducts similar or better recoveries of species of most all major lipid classes compared with the Folch method. The SAP method was applied to lipid extracts from complex biological samples, demonstrating that it can be powerfully utilized for fast (<3 min) preparation of lipids compared to the Folch method.