Identification of aromatic compounds existing in environmental, energy, and food samples are important for scientific and industrial researches and mass spectrometry (MS) is one of the most widely used analytical techniques to study. In this study, collisional cross section (CCS) values of a set of aromatic compounds were determined and the compounds were evaluated as calibration standards. The external calibration with poly-DL-alanine and internal and external calibration with the set of aromatic compounds were performed on the ion mobility mass spectrum obtained from crude oils. It was determined that external calibration method using the set of aromatic compounds is more accurate and easier method. The improved accuracy can be explained by structural similarity between the standards and the analytes compared to those of poly-DL-alanine.