Saliva often encountered at crime scenes is one of the most valuable resource for criminal identification or post-mortem interval estimation. However, the identification of saliva is quite challenging because most saliva has been found as a dry spot with very low concentration at crime scene. In addition, up to now, there is no any biosignature to differentiate human saliva from other human fluids and animal saliva. In previous study, we developed an analytical platform using glycomic approach to identify human saliva. We found that the presence of highly fucosylated N-glycans with significant abundances was saliva-specific feature to identify human saliva from other body fluids. Herein, we have developed and streamlined glycomics-based forensic platform using dried saliva spot (DSS) as a sampling technique for saliva samples. Briefly, dried saliva were prepared by spotting of human saliva on a protein saver card. N-glycans were released by PNGase F from saliva and enriched by PGC-SPE. Then, saliva N-glycans were characterized by nano LC/Q-TOF MS. In order to apply to forensic field, we compared N-glycans from liquid and dried saliva. Fifty N-glycan compositions were commonly identified and represent high correlation (R = 0.96). Additionally, highly fucosylated N-glycans, specific feature of human saliva, were equally observed from glycan profiles in DSS with similarity even in a trace amount. These studies can be useful to expand glycomic-based method to forensic science.